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Thus, it seems that for H.

Despite its expression in urediospores and germ tubes a slight increase observed in the expression of the MAPK during appressorium formation may suggest that this protein is important in signalling mechanisms leading to the formation of that structure Azinheira, Both polymers are regularly distributed over the walls of pre-penetration fungal structures Silva et al. Over the cell walls of intercellular hyphae a regular labelling for b -1,3-glucans was observed, while chitin accumulated preferentially over the internal part, likely to be less exposed to the eventual action of host chitinases Silva et al.

For different rusts, Deising et al. They suggested that restriction of chitin exposition to fungal structures which are not in direct contact with inter- and intracellular host defence enzymes may be a general rule in rust fungi. The ultrastructure of intercellular hyphae and haustoria of H. Cytochemical studies made by Silva et al. The first race differentiation of H.

No other studies were made on the physiological specialization of H. Molecular studies to detect genetic diversity in H. However, a linkage between the molecular markers obtained and the pathotypes used was not established.

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The inheritance studies of rust resistance carried out at CIFC have demonstrated that the gene-for-gene theory is applicable to coffee-rust interactions Noronha-Wagner and Bettencourt where the resistance of coffee plants is conditioned by at least nine major dominant genes S H 1-S H 9 , singly or associated.

By the same theory, it was possible to infer 9 genes of virulence v1-v9 in H. Rodrigues Jr. Besides these S H genes it is likely that other major and minor genes might condition the coffee-rust interactions Bettencourt and Rodrigues Jr. The coffee genotypes are classified in physiological groups which are distinguished from each other essentially by responses involving either complete resistance or susceptibility low and high infection type to several rust races.


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Group A, characterized by resistance to all the known rust races, has been found in hybrids between C. Plants of group A have also been found in C.

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D'Oliveira and Rodrigues Jr. Non-specific polygenic resistance has been assessed at CIFC and more extensively in other countries Kushalappa and Eskes , mainly under laboratory conditions using different parameters, such as latency period, percentage of sporulating lesions, and spore production per lesion. However, it was after the creation of CIFC that coffee breeding for rust resistance received a decisive impulse. Remarkably, most of the HDT offspring offered resistance to all or some of the known rust races. It has been demonstrated that HDT is supposed to be a natural hybrid between C.

This interspecific hybrid has the same number of chromosomes as C. The fundamental and applied research developed at CIFC on leaf rust, as well as the research it originated in several coffee-growing countries, led to substantial advances towards obtaining durable resistance to this disease in Arabica.

The hybrids H, H and H were introduced in the American Continent in , and F3 and further generations received the designations of Sarchimor, Cavimor and Cachimor Bettencourt, Catimor and Sarchimor, the most advanced selections, have been widely distributed in the coffee-growing countries, not only in Latin America but also in Africa Malawi , Asia India and Oceania Papua New Guinea.

Some genotypes of the referred coffee varieties, however, maintain their resistance and others, although infected in the field, present an incomplete type of resistance with others heavily infected, suggesting that they probably possess a polygenic type of resistance, like the variety Colombia Alvarado, However, it is interesting to note that when the yield is totally suppressed, the susceptible cultivar Caturra appears partially resistant under conditions of strong infection Bertrand et al.

Moreover in a F2 population resulting from a cross between 'Resistant x Susceptible', the same authors observed that plants with low productivity appear with very high frequency, partially or totally resistant and plants with high productivity appeared resistant or susceptible and rarely partially resistant. Consequently, some partial resistance might be explained by the physiological status of the plant.

Cytological and biochemical resistance mechanisms. There is no evidence for the existence of preformed defences in coffee, which could limit the growth of H. For a number of coffee Coffea spp.


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Cell death began to be observed around 2 days post-inoculation, in the guard cells only, or in both the guard and subsidiary cells at the infection sites in which the fungus reached the stage of appressorium or penetration hypha Silva ; Silva et al. Death of subsidiary and mesophyll cells invaded by a haustorium was observed from the 3rd day after inoculation. During the time course of infection, cell death spread to adjacent epidermal and mesophyll non-invaded cells, as has been generally described for other coffee resistant genotypes Martins et al.

Transmission electron microscope observation of host cells undergoing HR revealed membrane breakdown at the level of the plasma membrane and in different organelles, namely chloroplasts, nucleus and mitochondria, with a change in the chloroplast and nucleus appearance and coagulation of cytoplasm Silva et al. Another signal of incompatibility detected early at the cytological level was the haustoria encasement figure 1I. This host response was also observed in compatible interactions, but latter in the infection process from the 7 th day post-inoculation and only in a small number of haustoria Rijo and Vasconcelos, ; Silva, ; Silva et al.

The haustoria encased material in resistant or susceptible leaves reacted positively for callose and b -1,4-glucans, as indicated by the use of polyclonal antibodies raised against b -1,3-glucans and an exoglucanase-gold complex, respectively. The use of anti-galacturonic acid monoclonal antibodies JIM7 allowed the localization of pectins in the encasing material around the penetration pegs, but not around haustorial bodies Silva et al. In several plants resistant to rust and other obligate biotrophs, haustorium encasement has been regarded as one expression of incompatibility Littlefield and Heath ; Cohen et al.

Callose, the major compound of haustorial encasements, has been reported to be less permeable to small molecules than other cell wall components Heslop-Harrison, and may therefore restrict the passage of nutrients to the fungus and consequently to slow the fungal growth Rijo and Vasconcelos, Biochemical studies, with coffee resistant genotypes, revealed an early increase of phenylalanine ammonia-lyase PAL and peroxidase activity just before or at the same time as the beginning of the observation of cell death, which may indicate the involvement of these enzymes in HR.

By days post-inoculation a second increase of PAL and peroxidase activity was observed which can be related with the later accumulation of phenolic compounds and lignification of the host cell walls detected cytologically Silva et al. The isoenzyme pattern for peroxidases obtained by IEF gels showed an increase in activity of anionic isoenzymes and a new cationic isoform at the same time as the first peak of peroxidase activity detected in the incompatible interactions Silva et al. At that time, peroxidase activity, cytochemically localized using DAB Diaminobenzidine , was detected at the interface host cuticle-fungal pre-penetration structures, as well as in the walls, middle lamella, cytoplasmic contents, chloroplasts and endoplasmic reticulum of stomatal and spongy cells, at infection and penetration sites.

The treatment of resistant coffee leaves with 2,4-dichlorophenol, an activator of peroxidases and other oxidases, significantly increased cell death. On the contrary, salicyl hydroxamic acid, an inhibitor of the same enzymes and diphenyleneiodonium chloride, an inhibitor of NADPH oxidases decreased cell death. These results suggested that the peroxidases, NADPH oxidases and eventually other oxidases are involved in the HR of the coffee-rust interaction. The same kind of treatments using scavengers of active oxygen species catalase, superoxide dismutase and manitol showed that only the superoxide dismutase significantly inhibited the cell death, also suggesting the involvement of the superoxide anion radical O 2 - in the HR Silva et al.

On the other hand, studies made by Rojas et al. An early increase of chitinase and glucanase activity in coffee-leaf rust incompatible interactions, but not in the compatible ones was observed by Maxemiuc-Naccache et al. Similar results were obtained when studying chitinase activity in intercellular fluids IF of incompatible coffee-rust interactions. Although basic isoforms of chitinases, from IF of coffee leaves, were present in both compatible and incompatible interactions, they were detected earlier in the incompatible ones. Ultrastructural observations of different coffee resistant genotypes revealed the accumulation of a material partially crystallised in the intercellular spaces around the senescent hyphae, next to dead host cells and in close association with the middle lamella figure 1J , around days post-inoculation.

However, such material was never detected in healthy or susceptible tissue. Cyto- and immunocytochemical tests showed that at the beginning of accumulation the material contained weakly esterified pectins. It also contained polysaccharides and phenolic-like compounds. Cellulose, hemicellulose, extensins, hydroxyproline-rich glycoproteins and proteins were not detected.

Although the role of this material is unknown it might be the result of plant cell death associated with the slowdown of tissue invasion by the pathogen Silva et al. Another response observed, around 12 days post-inoculation in different coffee resistant genotypes was the hypertrophy of the host mesophyll cells in the infection area Rijo et al. These larger cells surrounding the intercellular hyphae gave rise to a localized tumefaction and corresponded macroscopically to the reaction type flt , the most common reaction type of incompatible coffee-rust interactions.


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  7. Molecular disease resistance responses of C. Identification of coffee genes expressed during the HR to H. To gain insights into defence and resistance gene activation in coffee, a catalogue of genes expressed early in coffee leaves when challenged by the rust pathogen was established Fernandez et al. Library 1 contained subtracted cDNAs obtained from coffee leaves inoculated with the rust fungus for 12 h.

    Library 2 contained subtracted cDNAs derived from a pool of mRNAs obtained from coffee leaves collected 24 and 48 h post-inoculation Fernandez et al. Genes associated with expression of early resistance mechanisms of coffee plants to parasites were isolated from the two cDNA libraries Fernandez et al. Although far from being exhaustive, the ESTs isolated may provide a significant set of data for improving our knowledge of coffee resistance to pathogens.

    A lot of the genes isolated showed homology to known plant genes suggesting conservation of signalling pathways and resistance mechanisms against pathogens in C. Evolution of the percentage of ESTs in these categories during infection may reflect reorganization of the plant host metabolism in order to fight off pathogen attack. It has been recently shown that a shift from housekeeping to pathogen defence metabolism in Arabidopsis thaliana correlated with induction of HR to Pseudomonas syringae Scheideler et al. Coffee gene expression in biotic and abiotic stresses. Coffee molecular responses to rust infection and to abiotic treatments were examined by semi-quantitative RT-PCR table 1.

    A set of candidate genes were chosen on the basis of their homology to known plant genes involved in resistance to parasites, and on the basis of a differential screening of the cDNA clones of the two libraries Fernandez et al. Among them were genes putatively encoding disease resistance signalling proteins, stress response proteins, disease resistance and defence proteins, transcription factors, as well as gene coding proteins of unknown function table 1.

    In addition, 9 genes also showed transient enhanced transcript accumulation during the incompatible interaction HR when comparing with the compatible interaction Fernandez et al. Gene expression patterns upon abiotic treatments showed that about half of the genes examined also responded to SA or to wounding. Based on sequence similarity to known genes, 4 out of the 9 HR-upregulated sequences putatively encoded stress-related proteins or components of disease resistance signalling pathways table 1.

    Cytochrome P genes may be involved in the biosynthesis of defence-related compounds, such as the Arabidopsis PAD 3 gene required for camalexin synthesis in the resistance response to Alternaria brassicicola Zhou et al. Recently, Kanzaki et al. With regard to HR-upregulated sequences encoding resistance-signalling components, two coffee sequences best matched the A.

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    Finally, 4 other HR-upregulated cDNA clones were of potential interest regarding defence mechanisms table 1. One EST putatively encoded a receptor-like kinase. This class of signal proteins is involved in a diverse array of developmental and defence functions Du and Chen, ; Morris and Walker, Another gene best matched an UDP-glucose: salicylic acid glucosyltransferase. Glucosyltransferases catalyze the transfer of glucose residues to numerous substrates and regulate the activity of compounds that play important roles in plant defence against pathogens, such as salicylic acid Chong et al.

    A number of gain-of-function studies have shown the direct implication of several transcription factors in potentiating the plant responses to pathogen infection.

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    Particularly involved are several WRKY proteins which are implicated in the regulation of several biological processes, including pathogen defence Dong et al. Time-course of gene induction during coffee rust infection. Real-time quantitative RT-PCR experiments were conducted to quantify gene expression levels during coffee rust infection. The Ubiquitin gene chosen as internal reference of gene expression was assayed in parallel with the candidate genes.